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  5. Thermo Scientific Chemicals HEPES-buffered saline, pH 7.0 (2X for transfection)

Thermo Scientific Chemicals HEPES-buffered saline, pH 7.0 (2X for transfection)

Catalog Number p-7042467
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Quantity:
250 mL
500 mL
7365-45-9
C8H18N2O4S
238.30
MFCD00006158
JKMHFZQWWAIEOD-UHFFFAOYSA-N
23831
CHEBI:42334
OCCN1CCN(CCS(O)(=O)=O)CC1
7365-45-9
C8H18N2O4S
238.30
MFCD00006158
JKMHFZQWWAIEOD-UHFFFAOYSA-N
23831
CHEBI:42334
OCCN1CCN(CCS(O)(=O)=O)CC1

Description

HEPES-buffered saline (2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonic acid, CAS # 7365-45-9) is a Good’s buffer used to maintain physiological pH.

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

  • HEPES-buffered saline is an ionic buffer with a pKa=7.55
  • HEPES is an ethanesulfonic acid with a 4-(2-hydroxyethyl)piperazin-1-yl substitution within one of its methyl groups

Application

  • HEPES-buffered saline can be used to maintain physiological pH in a solution; it is frequently used for protein isolation and other molecular applications
  • Useful for cell culture, HEPES maintains pH even in the presence of carbon dioxide
  • It is intended for use in cell culture and molecular biology
TRUSTED_SUSTAINABILITY
Concentration 2X
pH 7.0
Physical Form Liquid
Quantity 250 mL
Solubility Information Miscible with water.
IUPAC Name 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
Chemical Name or Material HEPES-buffered saline

RUO – Research Use Only

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