Gata-3 Rat anti-Human, Mouse, Porcine, Rhesus Monkey, Alexa Fluor® 488, Clone: TWAJ, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 53-9966-42
Additional Details : Weight : 0.23750kg
DescriptionPlease refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|HDR, MGC2346, MGC5199, MGC5445|
|Human, Mouse, Porcine, Rhesus Monkey|
|Flow Cytometry, Immunocytochemistry, Immunofluorescence|
|Alexa Fluor™ 488|
|4° C, store in dark, DO NOT FREEZE!|
|14462, 2625, 713840, 733631|
For Research Use Only.