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FOXP3 Monoclonal Antibody (236A/E7), eFluor™ 450, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 48-4777-41
Additional Details : Weight : 0.23750kg
Description
Description: The 236A/E7 antibody reacts with human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3 Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25^bright population. The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). Applications Reported: This 236A/E7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 236A/E7 antibody has been pre-titrated and tested using the Foxp3/Transctiption Factor Staining Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 μL (0.25 μg) per test.
FOXP3 Monoclonal antibody specifically detects FOXP3 in Human, Non-human Primate, Rhesus Monkey samples. It is validated for Flow Cytometry.Specifications
| FOXP3 | |
| Monoclonal | |
| 5 μL/Test | |
| PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
| Q9BZS1 | |
| Foxp3 | |
| Affinity chromatography | |
| RUO | |
| 50943, 574303 | |
| 4° C, store in dark, DO NOT FREEZE! | |
| Liquid |
| Flow Cytometry | |
| 236A/E7 | |
| eFluor 450 | |
| Foxp3 | |
| AIID; DIETER; forkhead box P3; forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; forkhead/winged helix transcription factor 3; Foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; regulatory protein Foxp3; RGD1562112; RP23-54C14.1; scurfin; scurfy; sf; XPID | |
| Mouse | |
| 25 Tests | |
| Primary | |
| Human, Non-human Primate, Rhesus Monkey | |
| Antibody | |
| IgG1 κ |
For Research Use Only.