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Fluorescein-D22 Coactivator Peptide, 100μM

Fluorescein-D22 Coactivator Peptide, 100 μM

Brand:  Invitrogen PV4387

Additional Details : Weight : 0.50000kg

Product Code. 10036842

  • 7860.00 NOK / 1mL
Estimated Shipment: 08-08-2024
to see stock.

Description

Description

LanthaScreen™ fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays. Assays developed using these reagents enable primary or secondary screening of nuclear receptor agonists and/or antagonists.

How they work
Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events, including dissociation of repressor proteins, association of coactivator proteins, and assembly of pol II and other transcriptional factors for activation of target genes. TR-FRET based assays can be developed using the LanthaScreen™ panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding, either by determining the affinity of ligand-bound receptor for different coregulator peptides, or by identifying additional agonists or antagonists via displacement or recruitment of a specific coregulator peptide. In the LanthaScreen™ TR-FRET RXR beta Coactivator Assay, a terbium (Tb)-labeled anti-GST antibody is used to indirectly label GST-tagged RXR beta protein. An agonist (9-cis Retinoic Acid) added to the receptor which, upon ligand binding, causes a conformational change resulting in recruitment of the Fl-D22 coactivator peptide (Figure 1). The binding of the fluorescent coregulator peptide to RXR beta causes an increase in the TR-FRET emission ratio (Figure 2).

Format:
LanthaScreen™ fluorescent nuclear receptor coregulator peptides are supplied as 100μM solutions in peptide-appropriate buffers.
Specifications

Specifications

≥95% by HPLC
100 μm
Fluorescein-D22 Coactivator Peptide, 1mL, 100μM Concentration, 2499Da Molecular Weight, –5 to –30°C Storage Condition, Used for Pharma and Biopharma, Target and Lead Identification and Validation
2499Da
1 mL
Fluorescein- LPYEGSLLLKLLRAPVEEV
Fluorescein - D22 Coactivator Peptide
-5°C to -30°C
Pharma and biopharma, target and lead identification and validation
Fluorescein - D22 Coactivator Peptide
For research use only. Not for use in diagnostic procedures
10mM Na2CO3 (pH 11.0)
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