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Thermo Scientific™ FastDigest NdeI

The FastDigest NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FauNDI).

528.00 NOK - 10010.00 NOK

Specifications

Methylation Sensitivity Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Enzyme Nde I
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
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Product Code Brand Quantity Price Quantity & Availability  
10349709
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Thermo Scientific™
FD0583
100 μL (100 Reactions)
528.00 NOK
100 reactions
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Thermo Scientific™
FD0584
300 μL (300 Reactions)
1342.00 NOK
300 reactions
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11803843
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Thermo Scientific™
FD0585
2 x 500 μL (1,000 Reactions)
10010.00 NOK
1000 reactions
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Description

Description

Lambda DNA digested with NdeI, 0.7% agarose, 7 cleavage sites

FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Thermo Scientific FastDigest NdeI restriction enzyme recognizes CA^TATG site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: FauNDI.

Thermo Scientific FastDigest NdeI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment (Cat. No. EP0051), T4 DNA Ligase (Cat. No. EL0014), alkaline phosphatases and T4 DNA Polymerase (Cat. No. EP0061) all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features

• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.

Specifications

Specifications

Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
10X FastDigest Buffer/FastDigest Green Buffer
37°C
Restriction Enzyme
FastDigest™
65°C
Nde I
Yes
No
Traditional Cloning
5' protruding ends
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.