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COX2 Mouse anti-Human, PE-Cyanine7, Clone: COX 229, eBioscience

Mouse Monoclonal Antibody

Brand:  Invitrogen 25-9725-80

Additional Details : Weight : 0.01000kg

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Product Code. 16596990

  • 1582.00 NOK / 25µg

Estimated Shipment: 16-11-2021

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Description

Description

Description: This COX 229 monoclonal antibody recognizes human COX2 also known as PTGS2. This antibody has been reported to cross-react with the mouse and rat COX2. For optimal performance, we recommend using this antibody with the Foxp3 / Transcription Factor Staining Buffer Set and protocol. Applications Reported: This COX 229 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This COX 229 antibody has been tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product No. 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at <a href="https://www.thermofisher.com/flowprotocols">www.thermofisher.com/flowprotocols</a>. This may be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). It is constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and is up-regulated under pathological conditions, such as in cancer and inflammation (in contrast to the iso-enzyme PTGS1, which is expressed ubiquitously). Up-regulation of COX2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. COX2 is naturally inhibited by calcitriol (the active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo in part by interfering with transcription initiation and elongation. COX2 is a target of NSAID such as aspirin, which can reduce pain and swelling from inflammation driven by COX2.
Specifications

Specifications

COX2
Monoclonal
0.2 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P35354
PTGS2, prostaglandin-endoperoxide synthase 2, Cyclooxygenase-2, PHS II, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2
Mouse
IgG1, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
5743
Flow Cytometry
COX 229
PE-Cyanine7
PTGS2
Liquid
PTGS2
Synthetic peptide from human COX2
Affinity Chromatography
RUO
Antibody
Monoclonal
Human
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