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CD49d (Integrin alpha 4), PerCP-eFluor™ 710, clone: R1-2, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0492-80

Additional Details : Weight : 0.25000kg

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Product Code. 15371260

  • 1074.00 NOK / 25µg


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Description

Description

Description: The R1-2 monoclonal antibody reacts with mouse CD49d, the 150kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin b7 and binds to the Mucosal vascular Addressin (MAdCAM-1).Applications Reported: This R1-2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This R1-2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD49d (Integrin alpha 4)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q00651
269C wild type, CD49 antigen-like family member D, VLA-4 subunit alpha, antigen CD49D, alpha-4 subunit of VLA-4 receptor, ITGA4, ITGA-IV
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
R1-2
PerCP-eFluor 710
Itga4
Liquid
Itga4
IgG2b, kappa, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
16401
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