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CD45, PE-Cyanine5, clone: 30-F11, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 15-0451-82

Additional Details : Weight : 0.25000kg

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Product Code. 15360480

  • 1722.00 NOK / 100µg


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Description

Description

Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: The 30-F11 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD45
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P06800
Cluster designation 45, common leukocyte antigen, T220 and B220
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
30-F11
PE-Cyanine5
Ptprc
Liquid
Ptprc
IgG2b, kappa, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
19264
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