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CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), Functional Grade, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
1264.00 NOK - 3860.00 NOK
Specifications
Antigen | CD357 (AITR/GITR) |
---|---|
Clone | DTA-1 |
Concentration | 1 mg/mL |
Applications | Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen) |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15228667
|
Affymetrix eBioscience
16-5874-81 |
50 μg |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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15238667
|
Affymetrix eBioscience
16-5874-83 |
200 μg |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
||||||
Description
Description: The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naive mice, GITR is expressed predomitly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes. Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression. Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease. Applications Reported: This DTA-1 antibody has been reported for use in flow cytometric analysis and immunohistochemistry of frozen mouse tissue. It has also been reported for use in functional assays. Applications Tested: This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.03 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This DTA-1 antibody has been tested by immunohistochemistry of frozen tissue and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.
Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. It is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Specifications
CD357 (AITR/GITR) | |
1 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
PBS with no preservative; pH 7.2 | |
21936 | |
IgG2b κ | |
Affinity chromatography | |
Antibody |
DTA-1 | |
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen) | |
Functional Grade | |
Rat | |
Mouse | |
O35714 | |
Tnfrsf18 | |
Primary | |
4° C | |
Tnfrsf18 |
For Research Use Only.