CD326 (EpCAM), Biotin, clone: 1B7, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 13-9326-80
Additional Details : Weight : 0.25000kg
DescriptionDescription: EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1) is a 40 kD cell-surface adhesion molecule participating in homophilic, calcium-independent cell-cell interactions. EpCAM is a type-I transmembrane protein, and is expressed primarily on the basolateral surface of most epithelia. Although normal epithelia express low levels of EpCAM, increased expression has been correlated with increased proliferation and progression to a mesenchymal phenotype. EpCAM has also been used as a diagnostic marker on circulating metastatic carcinoma cells, while cancer cells of non-epithelial origin do not express EpCAM.Applications Reported: This 1B7 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: This 1B7 antibody has been tested by flow cytometric analysis of the A549 cell line. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This 1B7 antibody has been tested by immunohistochemistry of frozen tissue and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Epithelial Specific Antigen, ESA, KSA, M4S1, MK-1, DIAR5, EGP-2, EGP40, KS1/4, MIC18, TROP1, TACSTD1, EPCAM, Epithelial cell adhesion molecule|
|Flow Cytometry, Immunohistochemistry (Frozen)|
|IgG1, kappa, kappa|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.