missing translation for 'onlineSavingsMsg'
Learn More

CD29 (Integrin beta 1), PerCP-eFluor™ 710, clone: eBioHMb1-1 (HMb1-1), eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0291-80

Additional Details : Weight : 0.25000kg

 View more versions of this product

Product Code. 15361240

  • 848.00 NOK / 25µg


In Stock 4
Add to basket

Description

Description

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties of beta1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most beta1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of beta1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a beta1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta.
Specifications

Specifications

CD29 (Integrin beta 1)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P09055, P49134
FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB, ITGB1, ITBG1D
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse, Rat
Flow Cytometry
eBioHMb1-1 (HMb1-1)
PerCP-eFluor™ 710
Itgb1
Liquid
Itgb1
IgG
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16412, 24511
Videos

For Research Use Only.