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CD279 (PD-1) Monoclonal Antibody (J116), Functional Grade, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
1178.00 NOK - 19860.00 NOK
Specifications
Antigen | CD279 (PD-1) |
---|---|
Clone | J116 |
Concentration | 1 mg/mL |
Applications | Flow Cytometry, Functional Assay |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15238857
|
Affymetrix eBioscience
16-9989-80 |
25 μg |
1178.00 NOK
25µg |
Estimated Shipment: 31-05-2024 Log in to see stock. |
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15248857
|
Affymetrix eBioscience
16-9989-82 |
100 μg |
3095.00 NOK
100µg |
In Stock Log in to see stock. |
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15587836
|
Affymetrix eBioscience
16-9989-38 |
5 mg |
19860.00 NOK
5mg |
Estimated Shipment: 30-04-2024 Log in to see stock. |
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Description
Description: The J116 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predomitly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Binding of the J116 monoclonal antibody inhibits PD-1 signal transduction, however, it does not block binding of the ligand PD-L1. Applications Reported: This J116 antibody has been reported in in vitro functional assays. Applications Tested: The J116 antibody has been tested by flow cytometric analysis of human PD-1 transfected cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "e;danger signals"e; such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.Specifications
CD279 (PD-1) | |
1 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
PBS with no preservative; pH 7.2 | |
CD279; EGK_05005; hPD1; hPD-1; hPD-l; hSLE1; Ly101; mPD-1; PD1; PD-1; Pdc1; Pdcd1; programmed cell death 1; programmed cell death 1 protein; programmed cell death protein 1; programmed cell death protein 1-like; programmed death 1; Protein PD1; protein PD-1; sCD279; SLEB2; soluble CD279; systemic lupus erythematosus susceptibility 2 | |
Pdcd1 | |
Primary | |
4° C | |
Pdcd1 |
J116 | |
Flow Cytometry, Functional Assay | |
Functional Grade | |
Mouse | |
Human | |
Q15116 | |
5133 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |
For Research Use Only.