missing translation for 'onlineSavingsMsg'
Learn More

CD235a (Glycophorin A) Mouse anti-Human, Super Bright 600, Clone: HIR2 (GA-R2), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 63-9987-41

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 15943584

  • 1590.00 NOK / Pack of 25
Estimated Shipment: 08-12-2022
to see stock.

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Description: The 2B11 monoclonal antibody reacts with mouse and human CXCR4, also known as CD184, Fusin, LESTR, or HUMSTR. Fusin is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. In addition it has been shown to play a role in lymphocyte migration. The ligand for CXCR4 is SDF1. CXCR4 is required for maintece of granulocyte progenitors in the bone marrow and essential for B cell development. Expression of CXCR4 is different between mice and human. In the thymus CXCR4 is restricted to CD4+CD8+ cells while in the spleen predomit expression is found on B lymphocytes. The monoclonal antibody 2B11 recognizes the amino terminus of CXCR4 and has been reported to have functional activity; it does not block SDF1a but causes internalization of the receptor. Applications Reported: This 2B11 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 2B11 antibody has been tested by flow cytometric analysis of mouse thymocytes. This may be used at less than or equal to 1.0 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. Glycophorins A (GPA) and B (GPB) which are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.
Specifications

Specifications

CD235a (Glycophorin A)
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P02724
Mouse
25 Tests
Primary
Human
Antibody
IgG2b κ
Flow Cytometry
HIR2 (GA-R2)
Super Bright 600
GYPA
GYPA
Affinity chromatography
RUO
2993
4° C, store in dark, DO NOT FREEZE!
Liquid
Videos
SDS
Documents
Certificates
Special Offers

Special Offers