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CD20, eFluor 615, clone: L26, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 42-0202-80

Additional Details : Weight : 0.25000kg

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Product Code. 15371090

  • 988.00 NOK / 25µg


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Description

Description

Description: The monoclonal antibody L26 recognizes human CD20, a two transmembrane spanning protein found on precursor and mature B lymphocytes. Expression is lost upon differentiation to plasma cells. The levels of CD20 are critical in evaluating malignant B lymphocytes including Non-Hodgkin's Lymphoma, and Acute B cell, Chronic Lymphocytic and Hairy-Cell Leukemias. CD20 plays a role in B cell differentiation and activation. The structure of the protein shows a small extracellular loop with the carboxy and amino termini in the cytoplasmic compartment. The L26 antibody recognizes an epitope in the cytoplasmic domain and therefore requires permeabilization of the tissue for staining applications. Unlike many anti-CD20 antibodies that recognize the extracellular domain, the epitope recognized by L26 will not be blocked by therapeutic antibodies to CD20, like Rituximab.Applications Reported: This L26 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded (FFPE) tissue sections (IHC-P).Applications Tested: This L26 antibody has been tested immunohistochemistry on FFPE human tonsil with low pH antigen retrieval. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor™ 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD20
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P11836
B1, Bp35, CD20, LEU-16, MGC3969, MS4A1
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Cynomolgus Monkey, Human, Rhesus Monkey
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
L26
eFluor 615
MS4A1
Liquid
MS4A1
IgG2a, kappa, kappa
25μg
4° C, store in dark, DO NOT FREEZE!
Primary
102118491, 696843, 931
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