CD178 (Fas Ligand), PerCP-eFluor™ 710, clone: MFL3, eBioscience™
Armenian Hamster Monoclonal Antibody
Brand: Affymetrix eBioscience 46-5911-82
Additional Details : Weight : 0.25000kg
DescriptionDescription: The MFL3 monoclonal antibody reacts with mouse Fas (CD95) Ligand, a 40kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by mouse activated T cells. The interaction of FasL with its receptor CD95 induces Fas-mediated killing. It has been reported that the human FasL antigen is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26kDa soluble form. The degree of sensitivity for the mouse antigen to MMPs has not been reported.Applications Reported: This MFL3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MFL3 antibody has been tested by flow cytometric analysis of transfected cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|FasL, apoptosis antigen ligand 1, CD95 ligand, CD95L, CD178, APO1, Fas Ligand, Fas-LG|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.