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CD11a (LFA-1alpha), PerCP-eFluor™ 710, clone: M17/4, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0111-80

Additional Details : Weight : 0.25000kg

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Product Code. 15361230

  • 956.00 NOK / 25µg


Estimated Shipment date
from Supplier 27-06-2018
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Description

Description

Description: The M17/4 monoclonal antibody reacts with mouse CD11a, the 180kDa integrin alpha L, also known as the lymphocyte function associated antigen-1 (LFA-1) alpha chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin beta 2), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1. CD11a is expressed by all leukocytes.Applications Reported: This M17/4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This M17/4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD11a
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P24063
CD11 antigen-like family member A, LFA-1 alpha, LFA-1A, antigen CD11A p180, lymphocyte function-associated antigen 1 alpha polypeptide, integrin alpha-L
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
M17/4
PerCP-eFluor 710
Itgal
Conjugated
Itgal
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
16408
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