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HIF1A Monoclonal Antibody (mgc3), Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen MA1516
Additional Details : Weight : 1.24740kg
Description
MA1-516 detects hypoxia-inducible factor 1 alpha (HIF-1 alpha) from human, non-human primate, bovine, mouse and porcine cells. This antibody does not cross-react with ARNT or the related HIF-2 alpha. MA1-516 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, immunocytochemistry, and gel shift procedures. By Western blot, this antibody detects an ∽116 kDa protein representing HIF-1 alpha after hypoxic induction in COS cells. Immunofluorescence staining of HIF-1 alpha in COS-7 cells with MA1-516 yields nuclear staining after exposing cells to 1% oxygen for 4 hours. In gel shift assays, MA1-516 has been successfully used only with mouse HIF-1 alpha. Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
HIF1A Monoclonal antibody specifically detects HIF1A in Bovine, Human, Mouse, Non-human Primate, Porcine samples. It is validated for Gel Shift, Immunocytochemistry, Immunoelectrophoresis, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot.Specifications
| HIF1A | |
| Monoclonal | |
| 1 mg/mL | |
| PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.4 | |
| Q16665, Q61221, Q9XTA5 | |
| HIF1A | |
| Human HIF-1 alpha amino acids 530-826. | |
| 100 μL | |
| Primary | |
| Bovine, Human, Mouse, Non-human Primate, Porcine | |
| Antibody | |
| IgG1 |
| Gel Shift, Immunocytochemistry, Immunoelectrophoresis, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot | |
| mgc3 | |
| Unconjugated | |
| HIF1A | |
| AA959795; ARNT interacting protein; ARNT2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF1; HIF1 alpha; HIF-1 alpha; HIF1a; hif-1a; hif1a.L; HIF1alpha; HIF-1alpha; HIF-1-alpha; HIF1-alpha; HIF1-alpha protein; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1 subunit alpha L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia inducible factor-1 alpha; hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1 alpha subunit; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L home | |
| Mouse | |
| Protein A | |
| RUO | |
| 15251, 281814, 3091, 396696 | |
| -20° C, Avoid Freeze/Thaw Cycles | |
| Liquid |