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Invitrogen™ mRNA Catcher™ PLUS Purification Kit

The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format.

4615.00 NOK - 35480.00 NOK

Specifications

Isolation Technology Oligo dT Affinity
Purification Time 1.5 hr. (Including sample homogenization)
Elution Volume 80 μL
Sample Type Cells, Tissue, Total RNA, Whole Blood
Final Product Type mRNA
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Includes: 1 96-well mRNA Catcher PLUS plate; 4mL 2X Lysis Buffer L20; 30mL Wash Buffer W15; 4mL Dilution Buffer DL1; 8mL Elution Buffer E3; 100μL 0.5M DTT (4°C)

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Description

Description

The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format. By taking advantage of Locked Nucleic Acid (LNA) technology, the mRNA Catcher PLUS Kit provides improved performance and a faster protocol, while preserving mRNA yield and purity.

How it works
mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA. The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding. The mRNA can either be eluted or directly reversed-transcribed to cDNA directly in the well. The mRNA Catcher PLUS method does not require centrifugation, precipitation, phase separation, or column filtration. In addition, the entire method is amenable to automation for streamlined, high-throughput expression profiling for large-scale processes. Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR.

• Automated protocols, high-throughput compatible
• Robust performance: high binding capacity per well (>80ng/well)
• Reproducible results: low CVs from well-to-well and plate-to-plate (∽ 2%)
• Optimized protocols: for small sample sizes (100 cells, 4 mg tissue, and 40 μL blood)
• Compatible plate design: may be used with most PCR cyclers

Order Info

Shipping Condition: Wet ice

Specifications

Specifications

Oligo dT Affinity
80 μL
mRNA
Robotic Liquid Handling Systems
Room Temperature
>80 ng
1.5 hr. (Including sample homogenization)
Cells, Tissue, Total RNA, Whole Blood
High-throughput Compatible
RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
Cells: 100–106
Tissue: 4–40 mg
Total RNA: 0.1–100 μg
Whole Blood: 40 μL
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.