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Invitrogen™ AKT (Total/Phospho) Multispecies InstantOne™ ELISA Kit
InstantOne ELISA
Brand: Invitrogen™ 85-86046-11
4537.20 NOK valid until 2024-03-30
Use promo code "21648" to get your promotional price.
Additional Details : Weight : 0.23750kg
Includes: User Manual
InstantOne™ Assay Plate - 96 well (sealed)
Cell Lysis Buffer (5X)
Enhancer Solution
Wash Buffer (10X)
AKT 1 (Total) Capture Antibody Reagent
phospho-AKT 1/2/3 (Ser473) Capture Antibody Reagent
AKT 1 (Total) Detection Antibody Reagent
phospho-AKT (Ser473) Detection Antibody Reagent
Positive Control Cell Lysate (lyophilized)
Detection Reagent (TMB Substrate)
Stop Solution
Plate Seal
Description
The InstantOne™ ELISA is specifically engineered for accurate measurement of human, mouse, and hamster total AKT 1 and phosphorylated AKT 1/2/3 (Ser473) in cell lysates. Detection of rat AKT 1 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
AKT (protein kinase B, PKB, RAS-alpha) is a 57kDa serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. AKT is activated by insulin, PI3K, IGF1 and various other growth and survival factors. AKT promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. The AKT pathway is a major target for cancer therpeutics where AKT signaling dysfunction has implicated in many types of cancer, including cancer syndromes known as phakomatoses.Specifications
P31749,P31751,Q9Y243 | |
ELISA Kit | |
Human, Mouse, Rat | |
Colorimetric Microplate Reader | |
AKT,CWS6,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA,HIHGHH,PKBB,PKBBETA,PRKBB,RAC-BETA,MPPH,MPPH2,PKB-GAMMA,PKBG,PRKBG,RAC-PK-gamma,RAC-gamma,STK-2 | |
HRP | |
RUO | |
2°C to 8°C | |
Human | |
1 hr. 10 min. |
Biotin | |
Cell Lysates | |
ELISA | |
10000, 207, 208 | |
Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal | |
96 Tests | |
Cell Lysate,20 μL | |
Protein Kinase B, PKB, RAC-alpha serine/threonine-protein kinase | |
1 hr. 20 min. |
For Research Use Only