Applied Biosystems

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Applied Biosystems™ MicroAmp™ Optical 8-Tube Strip with Attached Optical Caps, 0.2 mL

For tube strips with domed caps for PCR experiments using 1 or 2 strips that require the use of the 96-well tray (Cat. No. 4379983) on the ProFlex™, SimpliAmp™, and Veriti™ thermal cycler 96-well blocks.

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Applied Biosystems™ SYBR™ Green Universal Master Mix

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

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Specifications

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
Reaction Speed	Standard
Label or Dye	SYBR Green
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	SYBR™
Detection Method	SYBR

Applied Biosystems™ Region of Interest (ROI) and Background Plates, 96-well

Used to maintain the ViiA™ 7, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, and QuantStudio™ 12K Flex real-time PCR systems with 96-well (0.2 mL) blocks.

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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

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Specifications

Shipping Condition	Wet Ice
InhibitorToleranceSimple	Medium
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Multiplex Capability	Single-plex
Detection Method	SYBR
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Polymerase	Dual-Lock Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	DNA
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	PowerUP™, SYBR™

Applied Biosystems™ TaqMan™ Genotyping Master Mix

Optimized for end-point fluorescence detection in SNP genotyping applications.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Genotyping
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Genotyping Target	Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known)
Product Type	Genotyping Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7
Sample Type	DNA (Genomic)
Reaction Speed	Standard
Technique	SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation)
Product Line	TaqMan™

Applied Biosystems™ StepOnePlus™ Real-Time PCR System Spectral Calibration Kit (96 well)

Easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR™ Green I, VIC™/JOE™, and NED™/TAMRA™/ROX™ dyes on the StepOnePlus™ RT-PCR System.

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Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl₂

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

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Specifications

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase
Hot Start	Built-In Hot Start
Overhang	3'-A
Concentration	5 U/μL
Exonuclease Activity	5' - 3'

Applied Biosystems™ Protein Thermal Shift™ Dye Kit

This solution significantly reduces the time and cost associated with screening samples relative to currently available methods.

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Applied Biosystems™ POP-7™ Polymer for 3730/3730xl DNA Analyzers

POP-7™ Polymer is a separation matrix for the 3730 and 3730xl DNA Analyzers. POP-7™ Polymer is customized for DNA sequencing and fragment analysis.

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Specifications

Accuracy	98.5% up to 900 bases (for 50 cm capillary), 98.5% up to 700 bases (for 36 cm capillary)
Product Type	Polymer
For Use With (Application)	Standard Sequencing, Fragment Analysis (Denaturing)
Test Time	1 hr for 36 cm capillary (std. seq.), 2 hrs for 50 cm capillary (long read seq.)
Product Line	POP-7™

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Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix

TaqPath BactoPure Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors.

Pricing and Availability
Specifications

Content And Storage	Contains 2X TaqPath BactoPure Microbial Detection Master Mix that includes: • Hot-start AmpliTaq Fast DNA Polymerase • Heat-labile uracil-DNA glycosylase (UNG) • dNTPs (with dUTP) • ROX dye (passive reference) • Optimized buffer Store at 2°C to 8°C in the dark.
InhibitorToleranceSimple	Very High
For Use With (Application)	Microbial Detection
PCR Method	qPCR
Multiplex Capability	Up to 3-plex
Detection Method	Primer-probe
Includes	dUTP, UNG/UDG
Thermostability	72 hr., Assembled Reaction
Sensitivity	Down to 1 copy
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio12k Flex Dx, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, 7500 Fast Dx System, StepOne™ System
Sample Type	DNA
Reaction Speed	Fast or Standard
Target Specificity	cDNA or gDNA only
Product Line	TaqPath™

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	25 X
Description	Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase	ArrayScript™
Concentration	25X
PCR Method	1-step RT-qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Format	Tube
Product Type	One-Step RT-PCR Reagent
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Sample Type	RNA
Reaction Speed	Standard
Product Line	AgPath-ID™, Ambion™

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Applied Biosystems™ POP-4™ Polymer, for 3500/SeqStudio™ Flex

POP-4, POP-6, and POP-7 are conveniently offered in easy to use pouch packages. The POP-4 separation matrix is optimized for HID/forensic applications.

Pricing and Availability
Specifications

For Use With (Application)	Human Identification
Product Line	POP-4™

Applied Biosystems™ QuantStudio™ 5 Spectral Calibration Plate 1 (FAM™, VIC™, ROX™, TAMRA™, and SYBR™ dyes), 384-well

This plate establishes the pure dye spectra and multicomponent values for FAM™, VIC™, ROX™, TAMRA™, and SYBR™ dyes on Applied Biosystems™ QuantStudio™ 5 384-well real-time PCR system.

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Applied Biosystems™ QuantStudio™ Absolute Q™ Isolation Buffer

The QuantStudio Absolute Q Isolation Buffer is intended to supplement the materials supplied in the QuantStudio Absolute Q MAP16 Plate Kit.

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Applied Biosystems™ MicroAmp™ EnduraPlate™ Optical 96-Well Clear Reaction Plates with Barcode

Ideal solution for work that requires special handling and an even greater degree of durability for use with multi-instrument experiments

Pricing and Availability
Specifications

Skirt Style	Semi
Content And Storage	Store at room temperature.
No. of Wells	96
For Use With (Equipment)	Thermal Cycler, Genetic Analyzer, DNA Analyzer
Product Type	Microplate
Reaction Speed	Standard
Volume (Metric) Working	0.2 mL
Barcode	Includes Barcode
Color	Clear,Optical
Material	Polycarbonate,Polypropylene
Certifications/Compliance	Certified DNA/RNase Free
Volume (Metric) Well	0.3 mL
Product Line	MicroAmp

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Applied Biosystems™ MicroAmp™ Optical 8-Tube Strip with Attached Optical Caps, 0.2 mL

Applied Biosystems™ SYBR™ Green Universal Master Mix

Applied Biosystems™ Region of Interest (ROI) and Background Plates, 96-well

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Applied Biosystems™ TaqMan™ Genotyping Master Mix

Applied Biosystems™ StepOnePlus™ Real-Time PCR System Spectral Calibration Kit (96 well)

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl₂

Applied Biosystems™ Protein Thermal Shift™ Dye Kit

Applied Biosystems™ POP-7™ Polymer for 3730/3730xl DNA Analyzers

Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

Applied Biosystems™ POP-4™ Polymer, for 3500/SeqStudio™ Flex

Applied Biosystems™ QuantStudio™ 5 Spectral Calibration Plate 1 (FAM™, VIC™, ROX™, TAMRA™, and SYBR™ dyes), 384-well

Applied Biosystems™ QuantStudio™ Absolute Q™ Isolation Buffer

Applied Biosystems™ MicroAmp™ EnduraPlate™ Optical 96-Well Clear Reaction Plates with Barcode

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