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Modifying Enzymes

Modifying Enzymes

Proteins that have the ability to structurally alter macromolecules without being altered themselves. Useful for a variety of molecular biology procedures; products include DNA and RNA polymerases, phosphatases, kinases, nucleases, etc.
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1 – 12 of 12 results
1

Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)

Content And Storage -20°C
Form Solid
Product Type Proteinase K
Color White
For Use With (Application) Isolation of DNA and RNA from cell and tissue preparations, inactivating many Enzymes, including Endogenous Nucleases
Source Tritirachium album
2

OPTIZYME™ DNA Polymerase I, Fisher BioReagents™

Content And Storage -20°C
Form Liquid
Product Type DNA Polymerase I
Storage Buffer OPTIZYME™ DNA Pol I Buffer 10X : Tris HCl 500mM (7.5 pH at 25°C), MgCl2 100mM, DTT 10mM
Color Colorless
pH 7.5
Concentration 10 U/μL
For Use With (Application) DNA Polymerase I labels DNA by nick translation in conjunction with DNase I, second-strand cDNA synthesis in conjunction with RNase H, incorporation of modified Nucleotides, Reverse Transcriptases
Source E. coli cells with a cloned polA gene
3

OPTIZYME™ T4 DNA Polymerase, Fisher BioReagents™

Content And Storage -20°C
Form Liquid
Product Type T4 DNA Polymerase
Storage Buffer OPTIZYME™ T4 DNA Pol Buffer 5X: Tris HCl 335mM (8.8 pH at 25°C), MgCl2 33mM, DTT 5mM, (NH4)2SO4 84mM
Color Colorless
pH 7.5
Concentration 5 U/μL
For Use With (Application) Synthesis of labeled DNA probes, creation of blunt PCR products with 3'-dA overhangs, ligation-independent cloning of PCR products, Restriction Enzymes, reverse transcriptases and T4 DNA ligase
Source E. coli cells with a cloned gene 43 of bacteriophage T4
5

OPTIZYME™ Alkaline Phosphatase, Fisher BioReagents™

Content And Storage -20°C
Form Solid
Product Type Alkaline Phosphatase
Storage Buffer OPTIZYME™ AP Buffer 10X : Tris HCl 100mM (8 pH at 37°C), MgCl2 50mM, KCl 1M, Triton™ X-100 0.2%, BSA 1mg/mL
Color Colorless
pH 7.4
Concentration 1 U/μL
For Use With (Application) DePhosphorylates cloning vector DNA (10 min. at 37°C), fast thermo-inactivation (5 min. at 75°C), Nucleotide degradation prior to sequencing PCR products, active on Nucleotides, Protein dephosphorylation, restriction En
Source E. coli cells with a cloned bacterial Alkaline Phosphatase gene
6

Fisher BioReagents™ Leupeptin Hemisulfate

(Acetyl-Leu-Leu-Arg-al), Valeramide

Content And Storage -20°C
Form Chunks, Powder
Product Type Leupeptin Hemisulfate
Molecular Weight (g/mol) 475.60
Color White
For Use With (Application) Inhibitor for Trypsin, Plasmin, Papain, Cathepsin B
7

OPTIZYME™ M-MLV Reverse Transcriptase, Fisher BioReagents™

Content And Storage -20°C
Form Liquid
Product Type OPTIZYME™ M-MLV Reverse Transcriptase
Storage Buffer OPTIZYME™ M-MLV RT Buffer 5X : Tris HCl 250mM (8.3 pH at 25°C), KCl 250mM, MgCl2 20mM, DTT 50mM
Color Colorless
pH 8.3
Concentration 200 U/μL
For Use With (Application) Generation of labeled cDNA probes for microarrays, synthesis of cDNA for cloning and expression, analysis of RNA by primer extension, DNA labeling, optimum activity at 42°C, incorporates modified Nucleotides
Source E. coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus Reverse Transcriptase
8

Fisher BioReagents™ Lysozyme, Egg White (White Cryst. Powder)

Content And Storage <0°C
Form Crystalline Powder
Product Type Lysozyme
Color White
For Use With (Application) Used to lyse E. coli for preparation of plasmid DNA from transformed cell cultures
Source Egg white
9

OPTIZYME™ T4 DNA Ligase, Fisher BioReagents™

Content And Storage -20°C
Form Liquid
Product Type T4 DNA Ligase
Storage Buffer OPTIZYME™ T4 DNA Ligase Buffer 10X: Tris HCl 400mM, MgCl2 100mM, DTT 100mM, ATP 5mM (7.8 pH at 25°C), OPTIZYME™ PEG 4000 Solution 50%: Polyethylene Glycol 50% (w/v)
Color Colorless
pH 7.5
Concentration 5 U/μL
For Use With (Application) Used to clone restriction Enzyme generated DNA fragments, cloning PCR products, ligation of double-stranded Oligonucleotide linkers or adaptors to DNA, site-directed mutagenesis, amplified fragment length polymorphism, ligase-mediated RNA detection, nick
Source E. coli cells with a cloned gene 30 of bacteriophage T4