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PCR Equipment and Supplies

PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.
PCR Supplies (190)
Thermal Cyclers (12)
Oligonucleotides (5)
Thermal Cycler Accessories (1)

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1 – 15 of 123 results
1

Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix
GREENER_CHOICE

AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.

EDGE
Shipping Condition Room Temp or Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Overhang 3'-A
Green Features Sustainable packaging
Concentration 2X
For Use With (Application) Cloning,Hot-start PCR
Exonuclease Activity 5' - 3'
Polymerase AmpliTaq Gold 360 DNA Polymerase
Format Tube
Size (Final Product) 5 kb or less
Reaction Format SuperMix or Master Mix
Product Type PCR Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Starting Material DNA
2

Invitrogen™ Platinum™ Direct PCR Universal Master Mix
GREENER_CHOICE

Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.

EDGE
Shipping Condition Dry Ice
Polymerase Platinum II Taq Hot-Start DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Size (Final Product) 8 kb
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Universal Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang 3'-A
Green Features Less hazardous, less waste
Concentration 2X
For Use With (Application) Hot-start PCR,Real Time PCR (qPCR)
3

Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase
GREENER_CHOICE

The Applied Biosystems AmpliTaq Gold 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.

EDGE
Shipping Condition Room Temp or Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Overhang 3'-A
Green Features Sustainable packaging
Concentration 5 U/μL
For Use With (Application) Hot-start PCR
Exonuclease Activity 5' - 3'
Polymerase AmpliTaq Gold 360 DNA Polymerase
Format Tube
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Reaction Speed Standard
Hot Start Built-In Hot Start
Starting Material DNA
4

Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
GREENER_CHOICE

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

EDGE
GC-Rich PCR Performance High
Format Tube
Product Type 1-Step qPCR kit
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Mammalian Cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Green Features Fewer resources used and less waste, Less hazardous
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line Cells-to-CT™, TaqMan™
Detection Method TaqMan
5

Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit
GREENER_CHOICE

The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2X
Green Features Fewer resources used, less waste, less hazardous
Concentration 2X
For Use With (Application) Gene Expression
High-throughput Compatibility High-throughput Compatible
PCR Method 2-step RT-qPCR
Detection Method TaqMan
Format Tube
Product Type Fast Advanced Cells-to-Ct Kit
Sample Type Mammalian cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Purification Target Lysate
Starting Material 10-100,000 Cells
Product Line Cells-to-CT™
6

Applied Biosystems™ TaqMan™ MicroRNA Assay
GREENER_CHOICE

Applied Biosystems TaqMan MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with an miRNA-specific primer, followed by real-time PCR with TaqMan probes.

Shipping Condition Room Temperature
GC-Rich PCR Performance High
Content And Storage • 1 tube containing RT primer at concentration of 5X (XS and S sizes), 20X (M size), or 60X (L size)
• 1 tube containing a 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.
Format Tube
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Reaction Speed Fast
Green Features Sustainable packaging
PCR Method qPCR
Product Line TaqMan™
7

TaqMan® TaqMan™ Gene Expression Assay (FAM)
GREENER_CHOICE

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.

Promotions are available
Shipping Condition Room Temperature
GC-Rich PCR Performance High
Content And Storage 1 tube containing 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15°C to -25°C.
Fidelity (vs. Taq) 60 X
Product Type Gene Expression Assay
Reaction Speed Fast
Green Features Sustainable packaging
PCR Method qPCR
Product Line TaqMan™
Detection Method Primer-probe
8

Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents
GREENER_CHOICE

TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.

Promotions are available
GC-Rich PCR Performance Low
Format Tube(s)
Internal Probe Modification TAMRA™ Quencher (3'), VIC™ (5')
Product Type Exogenous Internal Positive Control Reagent
Reaction Speed Standard
Green Features Sustainable packaging
PCR Method qPCR
Product Line TaqMan™
9

TaqMan® TaqMan™ Gene Expression Assay, VIC
GREENER_CHOICE

Applied Biosystems TaqMan Gene Expression assays, VIC, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5' end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3' end.

Promotions are available
GC-Rich PCR Performance High
Fidelity (vs. Taq) 60 X
Format Tube
Product Type Gene Expression Assay
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Reaction Speed Fast
Green Features Sustainable packaging
PCR Method qPCR
Detection Method Primer-probe
10

Applied Biosystems™ TaqMan™ Copy Number Reference Assay, human, RNase P
GREENER_CHOICE

Human TaqMan™ Copy Number Reference Assays are run with human TaqMan™ Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome.

Promotions are available
Shipping Condition Ambient Temperature
GC-Rich PCR Performance Low
Content And Storage Control Primer-Probe Set(s)
Purity or Quality Grade RNase-Free, DNase-Free
Internal Probe Modification TAMRA™ Quencher (3'), VIC™ (5')
Form Frozen
Gene Symbol RPPH1
Green Features Sustainable packaging
Concentration 20X
PCR Method qPCR
Detection Method Primer-probe
Target RNase P
Purification Method HPLC
Format Tube
Product Type Configurable Assay
Reaction Speed Standard
5'Primer Modification None
Label or Dye VIC
3'Primer Modification None
Species Human
Product Line TaqMan™
11

Applied Biosystems™ TaqMan™ Copy Number Reference Assay, mouse, Tfrc
GREENER_CHOICE

Mouse TaqMan™ Copy Number Reference Assays are run with predesigned, Custom Plus, and Custom TaqMan™ Copy Number Assays in a duplex, real-time PCR reaction to detect and measure copy number variations in the mouse genome.

Promotions are available
Format Tube
PCR Method qPCR
Detection Method Primer-probe
12

Applied Biosystems™ TaqMan™ MGB Probe
GREENER_CHOICE

Custom TaqMan™ Probe

Promotions are available
Shipping Condition Room Temperature
Content And Storage 1 tube containing a dual-labeled probe in solution.

Store at -15°C to -25°C
Purity or Quality Grade HPLC
Internal Probe Modification None
Form Liquid
Green Features Sustainable packaging
For Use With (Application) Real Time PCR (qPCR)
Purification Method HPLC
Format Tube
Product Type TaqMan Probe
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
5'Primer Modification FAM, NED, TET, VIC
3'Primer Modification MGB-NFQ
Product Line TaqMan™
13

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit
GREENER_CHOICE

The Ambion Single Cell-to-CT™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells.

Promotions are available
Shipping Condition Dry Ice
Sample Type Mammalian Cells
Reaction Speed 7 min.
Purification Time 7 min.
Green Features Fewer resources used and less waste, less hazardous
Starting Material 1-10 Cells
For Use With (Application) RT-PCR
PCR Method 2-step RT-qPCR
Product Line Ambion™, Cells-to-CT™
Detection Method TaqMan
14

Eppendorf™ Mastercycler™ X40
GREENER_CHOICE

Mastercycler X40 combines properties that raise your standard in end-point PCR. Classic features such as a 12-column gradient and ideal block temperature homogeneity are enhanced by an easy, intuitive user interface, a touchscreen and a protective SafeLid to protect your samples from evaporation.

Heated Lid 37°C to 110°C
Thermal Uniformity 20°C to 72°C ≤±0.2°C, 72°C to 95°C ≤±0.3°C
Block Configurations 96-well
Warranty 2 Years
User Interface Touchscreen
Dimensions 24.5 x 38.5 x 23 cm (9.7 x 15.2 x 9.1 in.)
Material Aluminum
No. of Programs 5000
Block Format Fixed
Gradient Range 1°C to 30°C
Weight 7.25 kg
Thermal Range 4°C to 99°C
Max. Heating Rate (Thermal Block) 3.3 °C/sec.
Product Type Thermal Cycler
Capacity 0.1 to 0.2 mL/Well
Max. Cooling Rate 1.5°C/sec.
Thermal Accuracy ±0.15°C
15

Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit
GREENER_CHOICE

The TaqMan™ Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Purity or Quality Grade UP (Ultra Pure)
Reverse Transcriptase M-MLV
Green Features Fewer resources used and less waste, Less hazardous
PCR Method 2-step RT-qPCR
Detection Method Primer-probe
Polymerase DNA Polymerase
Format Tube
Product Type Gene Expression Cells-to-C Kit
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Cell
Reaction Speed Standard
Product Line Ambion™, Cells-to-CT™, TaqMan™