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PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.
PCR Supplies (189)
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Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents
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TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.

EDGE
GC-Rich PCR Performance Low
Format Tube(s)
Internal Probe Modification TAMRA™ Quencher (3'), VIC™ (5')
Product Type Exogenous Internal Positive Control Reagent
Reaction Speed Standard
PCR Method qPCR
Product Line TaqMan
2
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Invitrogen™ Platinum™ Direct PCR Universal Master Mix
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Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.

EDGE
Shipping Condition Dry Ice
Polymerase Platinum II Taq Hot-Start DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Size (Final Product) 8 kb
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Universal Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 2X
For Use With (Application) Hot-start PCR,Real Time PCR (qPCR)
3
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Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)
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Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)

EDGE
Shipping Condition Room Temperature
Content And Storage Control Primer-Probe Set(s)
Gene Symbol 18S
Amplicon Size 187
Concentration 20X
For Use With (Application) Gene Expression
PCR Method 2-step RT-qPCR
Detection Method Primer-probe
Accession Number X03205.1
Target 18S rRNA
Format Tube
Label or Dye FAM
Species Eukaryotic, Rat, Mouse, Human
Product Line FAM, TaqMan
4
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Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
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Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

EDGE
GC-Rich PCR Performance High
Format Tube
Product Type 1-Step qPCR kit
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Mammalian Cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line Cells-to-CT, TaqMan
Detection Method TaqMan
5

Applied Biosystems™ Human PPIA (Cyclophilin A) Endogenous Control (VIC™/MGB probe, primer limited)
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Human PPIA (Cyclophilin A) Endogenous Control (VIC™/MGB probe, primer limited)

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6
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Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit
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The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2X
Concentration 2X
For Use With (Application) Gene Expression
High-throughput Compatibility High-throughput Compatible
PCR Method 2-step RT-qPCR
Detection Method TaqMan
Format Tube
Product Type Fast Advanced Cells-to-Ct Kit
Sample Type Mammalian cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Purification Target Lysate
Starting Material 10-100,000 Cells
Product Line Cells-to-CT
7
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Applied Biosystems™ TaqMan™ Gene Expression Assay (FAM)
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Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.

Shipping Condition Room Temperature
GC-Rich PCR Performance High
Content And Storage 1 tube containing 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15°C to -25°C.
Fidelity (vs. Taq) 60 X
Product Type Gene Expression Assay
Reaction Speed Fast
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
8
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Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix
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AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.

Shipping Condition Room Temp or Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Overhang 3'-A
Concentration 2X
For Use With (Application) Cloning,Hot-start PCR
Exonuclease Activity 5' - 3'
Polymerase AmpliTaq Gold 360 DNA Polymerase
Format Tube
Size (Final Product) 5 kb or less
Reaction Format SuperMix or Master Mix
Product Type PCR Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Starting Material DNA
9
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Applied Biosystems™ Custom TaqMan™ Gene Expression Assay, FAM
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Applied Biosystems Custom TaqMan Gene Expression assays are the custom version of our predesigned TaqMan assays for quantitative real-time PCR.

Shipping Condition Room Temperature
Content And Storage 1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.
Product Type Custom TaqMan Gene Expression
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
Product Line TaqMan
10

Applied Biosystems™ TaqMan™ Gene Expression Assay, VIC
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Applied Biosystems TaqMan Gene Expression assays, VIC, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5' end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3' end.

GC-Rich PCR Performance High
Fidelity (vs. Taq) 60 X
Format Tube
Product Type Gene Expression Assay
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Reaction Speed Fast
PCR Method qPCR
Detection Method Primer-probe
11

Applied Biosystems™ TaqMan™ MicroRNA Assay
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Applied Biosystems TaqMan MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with an miRNA-specific primer, followed by real-time PCR with TaqMan probes.

Shipping Condition Room Temperature
GC-Rich PCR Performance High
Content And Storage • 1 tube containing RT primer at concentration of 5X (XS and S sizes), 20X (M size), or 60X (L size)
• 1 tube containing a 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.
Format Tube
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Reaction Speed Fast
PCR Method qPCR
Product Line TaqMan
12
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Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (FAM™/MGB probe, non-primer limited)
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Human GAPD (GAPDH) Endogenous Control (FAM™/MGB probe, non-primer limited)

Shipping Condition Room Temperature
Content And Storage Control Primer-Probe Set(s)
Form Liquid
Gene Symbol GAPDH
Amplicon Size 122
Concentration 20X
For Use With (Application) Gene Expression
PCR Method 2-step RT-qPCR
Detection Method Primer-probe
Accession Number NM_002046.3
Target GAPDH
Format Tube
Label or Dye FAM
Species Human
Product Line FAM, TaqMan
13

Invitrogen™ Cells-to-CT™ Bulk Fast Advanced RT Reagents
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Invitrogen Fast Advanced Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription (RT) in the TaqMan Fast Advanced Cells-to-CT Kit and the SYBR Green Fast Advanced Cells-to-CT Kit.

14
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Applied Biosystems™ TaqMan™ MGB Probe
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Custom TaqMan probes are dual-labeled primers suitable for use in any real-time PCR application. They incorporate a 5' reporter dye and a 3' nonfluorescent quencher/minor groove binder (MGB). Choose among FAM, VIC, ABY, JUN, Cy5, TET, and NED as reporters.

Purification Method HPLC
Purity or Quality Grade HPLC
Format Tube
Internal Probe Modification None
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Form Liquid
3'Primer Modification MGB-NFQ
For Use With (Application) Real Time PCR (qPCR)
Product Line TaqMan
15
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Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit
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The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Format Kit
Reverse Transcriptase SuperScript IV
Reaction Format Premixed Components
Final Product Type First-Strand cDNA
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material Cells
For Use With (Application) RT-PCR,Real Time PCR (qPCR)