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Buffers and Standards

Buffers and Standards

Hydrogen ion buffers comprising a mixture of a weak acid and its conjugate base or vice-versa and used to stabilize pH; also diluents, washing solutions, and standard-value solutions used for a broad array of scientific calibration purposes.

The buffers used to calibrate pH meters may be certified and/or traceable to the National Institute of Standards and Technology (NIST). These buffers may also be color-coded for easy identification:

  • Red: pH 4.0
  • Yellow: pH 7.0
  • Blue: pH 10.0

 

Life Science Buffers (110)
General Chemistry Solutions (23)

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1 – 15 of 73 results
1

Fisher Bioreagents Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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PubChem CID 6503
CAS 77-86-1
Molecular Weight (g/mol) 121.136
ChEBI CHEBI:9754
SMILES C(C(CO)(CO)N)O
Synonym Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol
IUPAC Name 2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula C4H11NO3
3

Fisher Bioreagents PBS Tablets, Phosphate Buffered Saline, Fisher BioReagents™

Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!

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4

Fisher Bioreagents PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™

Commonly used buffer

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Content And Storage Room temperature
Form Liquid
5

Fisher Bioreagents Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™

Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.

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6

Fisher Bioreagents TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents

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Color Colorless
Physical Form Liquid
Chemical Name or Material Tris-EDTA
Grade Molecular Biology
DNase DNase free
Filtered Through Filtered through a 5-micron filter.
ChemAlert Storage Symbol Gray
Name Note 1X Solution, pH 8.0
CAS 7732-18-5
Protease Protease free
Health Hazard 3 Emergency Overview
May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention.

NFPA

Health:1
Flammability:0
Instability:0
Health Hazard 2 CAUTION!
pH 7.4 to 8.1
Synonym TE
Recommended Storage RT
7

Fisher Bioreagents HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents™

Commonly used buffering agent

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Color Undesignated
Physical Form Liquid
Chemical Name or Material HEPES Buffer
Grade Molecular Biology
Identification Pass Test
DNase DNase free
Merck Index 15, 4689
Concentration 0.95 to 1.05 M
ChemAlert Storage Symbol Gray
Absorbance ≤0.01(0.1M solution) at 260nm,0.004 max. (0.1M solution) at 280nm
Name Note 1M Solution, pH 7.3
CAS 7732-18-5
Protease Protease free
pH 7.2 to 7.5
Synonym N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
Molecular Formula C8H18N2O4S
Formula Weight 238.197
8

Fisher Bioreagents TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™

Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT

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Name Note 50X Solution
Concentration or Composition (by Analyte or Components) Tris (24%), Acetic Acid (5.0%), and EDTA (<2%).
CAS 6850-28-8
Protease Protease free
Physical Form Liquid
Grade Electrophoresis
Synonym TAE
Purity Grade Notes DNase-, RNase- and Protease-Free
DNase DNase free
Recommended Storage RT
Filtered Through Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol Gray
9

Fisher Bioreagents TBS, Tris Buffered Saline, 10X Solution, pH 7.4, Molecular Biology, Fisher BioReagents™

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Boiling Point 100°C
Color Colorless
Physical Form Liquid
Chemical Name or Material Tris Buffered Saline
Grade Molecular Biology
DNase DNase free
ChemAlert Storage Symbol Gray
Name Note 10X Solution
Concentration or Composition (by Analyte or Components) 10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl.
CAS 7732-18-5
Protease Protease free
pH 7.5
Synonym TBS
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
10

Fisher Bioreagents HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™

Commonly used buffering agent.

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Molecular Weight (g/mol) 238.30
ChEBI CHEBI:42334
Color White
Chemical Name or Material HEPES
Grade Molecular Biology
SMILES OCCN1CCN(CCS(O)(=O)=O)CC1
Identification Pass Test
DNase DNase free
Merck Index 15, 4689
InChI Key JKMHFZQWWAIEOD-UHFFFAOYSA-N
ChemAlert Storage Symbol Gray
Assay Percent Range ≥99 %
PubChem CID 23831
Absorbance 0.01 max. (0.1M solution) at 280nm
Percent Purity ≥99%
CAS 7365-45-9
Protease Protease free
Health Hazard 3 Emergency Overview
Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. .

NFPA

Health:2
Flammability:1
Instability:1
MDL Number MFCD00006158
Health Hazard 2 WARNING!
Solubility Information Soluble in water
pH 5.0 to 6.5
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
Molecular Formula C8H18N2O4S
12

Fisher Bioreagents PBS, Phosphate Buffered Saline, 1X Solution, pH 7.4, Fisher BioReagents™

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Content And Storage Room temperature
Form Liquid
Packaging Type Poly Cube
pH 7.3 to 7.5
Melting Point 0°C
13

Fisher Bioreagents Ethylenediaminetetraacetic Acid (0.5M Solution/pH 8.0), Fisher BioReagents

CAS: 60-00-4 Molecular Formula: C10H16N2O8 Molecular Weight (g/mol): 292.24 MDL Number: MFCD00003541 InChI Key: KCXVZYZYPLLWCC-UHFFFAOYSA-N Synonym: edta,edetic acid,ethylenediaminetetraacetic acid,edathamil,versene,endrate,havidote,titriplex,edta acid,sequestrol PubChem CID: 6049 ChEBI: CHEBI:42191 IUPAC Name: 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid SMILES: OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

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PubChem CID 6049
CAS 60-00-4
Molecular Weight (g/mol) 292.24
ChEBI CHEBI:42191
MDL Number MFCD00003541
SMILES OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
Synonym edta,edetic acid,ethylenediaminetetraacetic acid,edathamil,versene,endrate,havidote,titriplex,edta acid,sequestrol
IUPAC Name 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid
InChI Key KCXVZYZYPLLWCC-UHFFFAOYSA-N
Molecular Formula C10H16N2O8
14

Fisher Bioreagents Tris-Glycine-SDS, 10X Solution (Electrophoresis), Fisher BioReagents™

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Color Undesignated
Physical Form Liquid
Chemical Name or Material Tris-Glycine-SDS
Grade Electrophoresis
Identification Pass Test
DNase DNase free
Filtered Through Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol Gray
Name Note 10X Solution
Concentration or Composition (by Analyte or Components) 0.25M Tris Base, 1.92M Glycine, and 1.0% (w/v) SDS.
CAS 151-21-3
Protease Protease free
Synonym TG-SDS
Purity Grade Notes DNase-, RNase- and Protease-Free
Recommended Storage RT
15

Fisher Bioreagents TE Buffer, Tris-EDTA, 100X Solution, pH 8.0, Molecular Biology, Fisher BioReagents™

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Name Note 100X Solution
CAS 7732-18-5
Protease Protease free
Health Hazard 3 Emergency Overview
Irritating to eyes and skin. Wear personal protective equipment. Ensure adequate ventilation. Avoid contact with skin, eyes and clothing. Avoid ingestion and inhalation. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Get medical attention immediately if symptoms occur. Do not induce vomiting.

NFPA

Health:2
Flammability:0
Instability:0
Health Hazard 2 WARNING!
Color Undesignated
Physical Form Liquid
Chemical Name or Material Tris-EDTA
Grade Molecular Biology
DNase DNase free
Recommended Storage RT
Filtered Through Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol Gray