Save antibody and see more in Western blotting and immunofluorescence experiments.

The Thermo Scientific Pierce Immunostain Enhancer enables significant antibody dilution and increases signal intensity from 3- to 12-fold. The ready-to-use enhancer for immunohistochemical staining does not add steps to the workflow because it is used to dilute the primary and secondary antibodies. The Thermo Scientific SuperSignal Western Blot Enhancer contains a membrane treatment reagent and a primary antibody diluent that increase both signal intensity and sensitivity 3- to 10-fold. This versatile kit works with PVDF and nitrocellulose membranes and is compatible with fluorescent, chromogenic and chemiluminescent detection.

The best combination to reveal your proteins in Western blotting.

Thermo Scientific SuperSignal Chemiluminescent HRP and Pierce ECL Western Blotting Substrates offer a broad range of sensitivity and duration for your Western blotting applications. The Thermo Scientific Pierce Secondary Antibodies are affinity-purified polyclonal antibodies with well-characterized specificity resulting in high specificity and low background. The Goat Anti- Mouse-HRP (GAM-IgG-HRP) is specific of mouse immunoglobulin classes (IgG, IgM, IgA) and Goat Anti-Rabbit-HRP (GAR-IgG-HRP) targets rabbit immunoglobulin (IgG).

Light on your scale.

Thermo Scientific SuperSignal Chemiluminescent HRP and Pierce ECL Western Blotting Substrates offer a broad range of sensitivity and duration for your Western blotting applications. Thermo Scientific SuperSignal Enhanced Molecular Weight Protein Ladders contain ready-to-use stabilized mixtures of eight recombinant proteins ranging in size from 20 to 150kDa. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies. The standards are compatible with nitrocellulose and PVDF membranes, multiple blocking buffers, and many others detection methods.

Perfect results in your Western blotting.

Thermo Scientific Nitrocellulose Membranes contain 100% nitrocellulose and are compatible with a variety of detection methods (e.g., chemiluminescence, chromogenic, fluorescence). The Thermo Scientific PVDF Membranes have high binding affinity for proteins and nucleic acids and are effective for Western, Southern and Northern blotting methods. These membranes are more resistant to discoloration and have better retention of adsorbed proteins than other types of membranes. Thermo Scientific SuperBlock Blocking Buffer TBS is a solution containing a protein that provides fast and effective blocking for Western blotting applications. Thermo Scientific Pierce Reversible Protein Stain Kits for Membranes are rapid and sensitive alternatives to Ponceau S stain for reversible detection of protein bands on nitrocellulose or PVDF membrane after transfer from polyacrylamide gels.

The best combination for short run time and resolution.

Thermo Scientific Precise and Pierce Protein Gels* are cast in a durable plastic cassette using a neutral pH buffer giving them a long shelf life. Gels are individually packed in an easy-to-open plastic pouch and are ready to run with no comb or tape to remove. Pierce Protein Gels use a special formulation to produce stronger, more resilient gels, making handling during and after electrophoresis easier. The Thermo Scientific PageRuler Prestained and Spectra Multicolor Broad Range Protein Ladders are a mixture of 10 stained recombinant proteins for use as size standards in protein electrophoresis and Western blotting. The range for Spectra Multicolor Broad Range Protein Ladder is 10 to 260kDa. The range for PageRuler Prestained Protein Ladder is 10 to 170kDa.

Thermo Scientific Pierce ECL Plus Substrate is acridan-based, generating acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce strong and sustained chemiluminescence and a robust fluorescent signal at 440nm that can be captured by CCD and fluorescence imagers.

Highlights:

• High sensitivity - detect targets down to the low-picogram level
• Long signal duration - sustained light output for as long as 5 hours
• Versatile - compatible with both NC and PVDF membranes and images, too
• Flexible - one substrate delivers both chemiluminescent and chemifluorescent signals