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OPTIZYME™ T4 DNA Ligase, Fisher BioReagents™

692.00 NOK - 1167.06 NOK

Concentration	5 U/μL
Color	Colorless
pH	7.5
For Use With (Application)	Used to clone restriction Enzyme generated DNA fragments, cloning PCR products, ligation of double-stranded Oligonucleotide linkers or adaptors to DNA, site-directed mutagenesis, amplified fragment length polymorphism, ligase-mediated RNA detection, nick
Storage Buffer	OPTIZYME™ T4 DNA Ligase Buffer 10X: Tris HCl 400mM, MgCl2 100mM, DTT 100mM, ATP 5mM (7.8 pH at 25°C), OPTIZYME™ PEG 4000 Solution 50%: Polyethylene Glycol 50% (w/v)

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Products 2

Product Code	Brand	Quantity	Price	Quantity & Availability
Product Code	Brand	Quantity	Price	Quantity & Availability
12807152 View Documents Promotion Details missing translation for 'viewProductCertificates'	Fisher BioReagents BP8099-1	100 U	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

12837152 View Documents Promotion Details missing translation for 'viewProductCertificates'	Fisher BioReagents BP8099-5	500 U	This item is currently unavailable or has been discontinued. View the product page for possible alternatives.		N/A

Description

OPTIZYME™ T4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Applications and Features:

OPTIZYME™ T4 DNA Ligase is used to clone restriction enzyme generated DNA fragments
Fast sticky end ligation (10min at room temperature)
Cloning PCR products
Ligation of double-stranded oligonucleotide linkers or adaptors to DNA
Site-directed mutagenesis
Amplified fragment length polymorphism
Ligase-mediated RNA detection
Nick repair in duplex DNA, RNA or DNA/RNA hybrids
Active in buffers for OPTIZYME restriction enzymes

Supplied with:

10X OPTIZYME T4 DNA Ligase Buffer : 400mM Tris-HCl, 100mM MgCl₂, 100mM DTT, 5mM ATP (pH7.8 at 25°C)
50% OPTIZYME PEG 4000 Solution: 50% (w/v) polyethylene glycol

Source: E. Coli cells with a cloned gene 30 of bacteriophage T4
Quality control:

Specifications

Concentration	5 U/μL
pH	7.5
Storage Buffer	OPTIZYME™ T4 DNA Ligase Buffer 10X: Tris HCl 400mM, MgCl2 100mM, DTT 100mM, ATP 5mM (7.8 pH at 25°C), OPTIZYME™ PEG 4000 Solution 50%: Polyethylene Glycol 50% (w/v)
Source	E. coli cells with a cloned gene 30 of bacteriophage T4
Form	Liquid

Color	Colorless
For Use With (Application)	Used to clone restriction Enzyme generated DNA fragments, cloning PCR products, ligation of double-stranded Oligonucleotide linkers or adaptors to DNA, site-directed mutagenesis, amplified fragment length polymorphism, ligase-mediated RNA detection, nick
Content And Storage	-20°C
Product Type	T4 DNA Ligase

Safety and Handling

Product Identifier

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