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OPTIZYME™ NsiI (Mph1103I) Fisher BioReagents™

Brand: Fisher BioReagents BP8058-1

Product Code. 11974812

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Description

5'...A T G C A^T...3'
3'...T^A C G T A...5'

Supplied with: 10X OPTIZYME Buffer 5
Conditions for 100% Activity:

1X OPTIZYME Buffer 5: 10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl₂, 100mM KCl and 0.1mg/ml BSA
Incubate at 37°C

Enzyme Activity in OPTIZYME buffers:

Storage Buffer:

10mM Tris-HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Re-cleavage:

More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with NsiI.

Compatible Ends: Alw21I, PstI, SdaI, SduI

Methylation Effects:

Dam: Never overlaps - no effect

Dcm: Never overlaps - no effect

CpG: Never overlaps - no effect

EcoKI: Never overlaps - no effect

EcoBI: May overlap - effect not determined

Specifications

Concentration	10 U/μL
pH	7.4
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Quantity	1000 U
Product Type	NsiI

Components	25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride
For Use With (Application)	>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I
Content And Storage	Keep container tightly closed
Cut Site	ATGCA.T
Form	Liquid

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