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Search results for "cloning"

1 – 15 of 202,838 results
1

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells

Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

Promotions are available
2

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells

Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

Promotions are available
EDGE
3

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells

Promotions are available
EDGE
Promoter SP6
Content And Storage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Kanamycin (KanR), Zeocin™ (ZeoR)
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
4

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, without competent cells

Provides a highly efficient, 5-minute cloning reaction for the direct insertion of proofreading polymerase-amplified, blunt-ended PCR products into a plasmid vector for sequencing

Promotions are available
Shipping Condition Dry Ice
Promoter T7, T3
Content And Storage Box 1:
• Topoisomerase I-activated pCR™4Blunt-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• T3, T7, M13 forward, and M13 reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
Vector TOPO Cloning Vectors
Product Line TOPO™
5

Invitrogen™ Zero Blunt™ PCR Cloning Kit, without competent cells

Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases

Promotions are available
Promoter T7
Content And Storage Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers.

Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt PCR
Bacterial or Yeast Strain Not Included
Vector Blunt DNA Cloning Vectors
Product Line TOPO™, Zero Blunt™
6

Corning™ PYREX™ Cloning Cylinders
GREENER_CHOICE

Create identical cells by surrounding a single cell or group of cells

Product Type Cloning Cylinder
Material Borosilicate Glass
Sterility Non-sterile
7

Thermo Scientific™ CloneJET PCR Cloning Kit

Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.

Promotions are available
EDGE
Antibiotic Resistance Bacterial Ampicillin (AmpR)
Format Kit
Sample Type PCR Products
Cloning Method Restriction Enzyme⁄ MCS, Blunt PCR
For Use With (Application) PCR Cloning
Vector pJET1.2/blunt
Product Line CloneJET
8

Bel-Art™ Cloning Cylinders

Manufactured from polystyrene. SP Scienceware™ Cloning Cylinders facilitates the isolation of individual cell clones.

Product Type Cloning Cylinder
Material Polystyrene
Sterility Sterile
9

Invitrogen™ Zero Blunt™ PCR Cloning Kit

Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases

Promotions are available
Promoter T7
Content And Storage Zero Blunt™ PCR cloning kits contain linearized pCR™-Blunt vector, ExpressLink™ T4 DNA ligase , 5X ExpressLink™ T4 DNA ligase buffer, control template, dNTPs, sterile water, and M13 forward and reverse primers. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt PCR
Bacterial or Yeast Strain TOP10
Vector Blunt DNA Cloning Vectors
Product Line Zero Blunt™
10

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells

Promotions are available
EDGE
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Product Line TOPO™
11

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells

Promotions are available
Promoter T7, SP6
Content And Storage Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Cloning Method TOPO™-TA
Vector TOPO-TA Vectors
Product Line TOPO™
12

Thermo Scientific™ CloneJET™ PCR Cloning Kit with DH10B Competent Cells

An advanced positive-selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any blunt or sticky-end DNA fragment can be cloned.

Promotions are available
Content And Storage Dry Ice (-80°C)
Description Bacterial/Yeast Strain: DH10B
For Use With (Application) Cloning (Blunt PCR, Restriction Enzyme ⁄ MCS)
13

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with TOP10F' E. coli

Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

Promotions are available
Content And Storage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Bacterial or Yeast Strain TOP10F´
Vector TOPO-TA Cloning Vectors
Product Line One Shot™
14

Invitrogen™ pLenti6/V5 Directional TOPO™ Cloning Kit

Directional TOPO Cloning site for rapid and efficient directional cloning of blunt-end PCR products

15

Thermo Scientific™ aLICator LIC Cloning and Expression Kit 1 (untagged)

Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.